Protocol Reference — FOXO4-DRI

Conservative Protocol

100-400 mcg/kg SC every other day for 2-4 weeks

Derived from commercial sources. No human efficacy validation.

Research-Aligned Protocol

5 mg/kg SC three times per week (Mon/Wed/Fri) for 1-4 weeks

Mirrors Baar et al. 2017 mouse protocol. Significantly higher exposure.

Observation Period

Minimum 30 days between treatment pulses

Allows assessment of senescent cell clearance effects before re-dosing.

Cycle Rationale

Self-limiting mechanism: target pool depletes as therapy works

Unlike most peptides, prolonged continuous dosing is not the goal. Pulse-then-observe.

Time to Effect

Mouse studies show visible changes (fur) within 3-4 weeks of treatment initiation

Systemic biomarker changes (testosterone, renal markers) observed within similar timeframe in mice.